FastQCFastQC Report
Fri 10 Feb 2017
ERR1147751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743568
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85580.31192957491850026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.19073702565418463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39030.14226000594845833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.060.887871
GTATCAA72100.057.1473771
TCAACGC88950.045.4787254
ATCAACG91950.044.0596123
CAACGCA92750.043.872015
AACGCAG96450.042.3740356
TATCAAC106250.038.521642
ACGCAGA112400.036.1492847
CGCAGAG115050.035.4148948
GTACATG131700.033.5010071
TACATGG136350.032.1556782
ACATGGG135500.031.918353
CATGGGT41150.031.80524
GCAGAGT128950.031.7357759
AGAGTAC120800.026.51507410-11
CATGGGG67950.026.4400834
GAGTACT51950.025.36075812-13
CAGAGTA127400.024.60454210-11
ATGGGGG33250.023.259325
AGTACTT58050.022.23471612-13
Produced by FastQC (version 0.11.5)