FastQCFastQC Report
Fri 10 Feb 2017
ERR1147750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882390
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109350.37937267337175057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63510.22033798340960106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55210.1915424352707302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85950.056.5895161
GGTATCA45650.051.5129131
TCAACGC111200.043.3297044
ATCAACG112650.042.9303933
AACGCAG112750.042.6812866
CAACGCA114200.042.0351875
TATCAAC128000.038.200352
ACGCAGA132400.036.3467867
CGCAGAG137600.035.014028
GTACATG143500.033.853071
TACATGG147950.032.7678642
ACATGGG144550.032.550973
GCAGAGT149300.032.34989
CATGGGG75300.027.9649334
AGAGTAC140900.027.05872710-11
CATGGGT36850.025.6664124
CAGAGTA145450.024.76057410-11
GAGTACT65950.024.21529612-13
AGTACTT69750.023.49295812-13
ATGGGGG36700.023.3400575
Produced by FastQC (version 0.11.5)