FastQCFastQC Report
Fri 10 Feb 2017
ERR1147749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4138869
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152030.3673225704896676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95350.23037694597243838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69790.16862094451406895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46850.067.350461
GTATCAA95650.064.4213261
TCAACGC126150.048.2404024
CAACGCA129550.046.8825155
ATCAACG131800.046.1724363
AACGCAG136650.044.4901436
TATCAAC139550.043.7782022
ACGCAGA161800.037.6114167
CGCAGAG170500.035.6534738
GCAGAGT189600.032.06189
AGAGTAC171050.027.8187110-11
GTACATG154250.026.7860411
CAGAGTA180750.025.5031310-11
TACATGG163350.024.9817162
GAGTACT85450.024.64116712-13
ACATGGG167000.023.901693
GTACTTT91250.023.14011814-15
AGTACTT92450.022.77542312-13
AGTACAT125300.021.55136712-13
TATACCG5250.021.5286545
Produced by FastQC (version 0.11.5)