FastQCFastQC Report
Fri 10 Feb 2017
ERR1147748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114228
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.26753949819965034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21380.1918817333615741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14460.12977595249805246No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14130.1268142606360637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.055.134111
GGTATCA14350.053.0884741
TCAACGC32950.042.778264
CAACGCA33750.041.5880365
AACGCAG34450.040.570366
ATCAACG34650.040.507833
ACGCAGA39700.035.355077
TATCAAC41500.034.3948442
CGCAGAG41900.033.4972158
GTACATG41850.031.8562561
GCAGAGT46650.030.341439
TACATGG43550.030.181052
ACATGGG41850.029.985913
CGTCTTA1006.1494575E-826.7622914-15
AGAGTAC43250.024.9575110-11
GAGTACT21350.024.9307812-13
GCACCGT2951.8189894E-1024.1929846
CAGAGTA45450.023.88029510-11
CATGGGT12800.023.6968384
CATGGGG21200.023.2847964
Produced by FastQC (version 0.11.5)