FastQCFastQC Report
Fri 10 Feb 2017
ERR1147747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604333
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75301.2460017904036351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54920.9087704957366219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36760.608273915208999No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.21924998303915225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11070.18317715564101247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.12807508443192742No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC7320.12112527364879959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30200.060.873681
GTATCAA51950.048.099631
TATCAAC65450.038.0780372
AACGCAG66650.037.306316
ATCAACG67450.036.775643
TCAACGC68000.036.4781884
CAACGCA69550.035.665235
GCTACGA700.00278917133.996951
ACGCAGA77350.032.222547
CGCAGAG78650.031.7655728
GCAGAGT83700.029.920089
TACATGG61550.028.9910132
GTACATG61350.028.8018171
CATGGGG35650.028.3658314
GAGTACT44550.028.2379312-13
ACATGGG62050.028.1845863
ATGGGGG19600.027.9214445
CAGAGTA82050.026.53216410-11
AGTACAC10200.025.6601163
AGAGTAC81250.025.2926810-11
Produced by FastQC (version 0.11.5)