FastQCFastQC Report
Fri 10 Feb 2017
ERR1147746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104867
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139761.2649486318262742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91600.8290590632175637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73140.6619801297350723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26500.23984787309241745No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18690.169160632003671No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11890.10761476268184315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93300.050.099631
GGTATCA54950.045.728951
AACGCAG114550.040.720546
TATCAAC120100.038.8883172
ATCAACG121100.038.5180633
TCAACGC122100.038.2513284
CAACGCA123900.037.6956185
ACGCAGA133700.034.888097
CGCAGAG134600.034.7369278
GCAGAGT144700.032.2300839
ATGGGGG26700.030.9739115
CATGGGG59700.030.2964534
GTACATG106150.029.7305091
TACATGG108350.028.9932922
ACATGGG107650.028.9607473
AGTACTT80650.026.81105612-13
GAGTACT78100.026.81053712-13
AGAGTAC142000.026.72675510-11
TTACGCA900.0095314226.4429573
CAGAGTA144400.024.98489410-11
Produced by FastQC (version 0.11.5)