FastQCFastQC Report
Fri 10 Feb 2017
ERR1147745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889104
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117851.3254917310010976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74940.8428710252118988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55420.6233241555543558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.24080422537745863No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11300.12709424319314724No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9860.11089816264463999No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.10887365257607659No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9200.1034749590599075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102950.043.6516271
GGTATCA65750.040.013481
AACGCAG120450.036.7905356
TATCAAC128000.034.899292
ATCAACG127800.034.8608173
TCAACGC129100.034.4637034
CAACGCA131050.033.950895
ACGCAGA137750.032.0836527
CGCAGAG138550.031.9395358
GCAGAGT148000.029.9805329
ATGGGTG8300.029.3827445
CATGGGT16650.027.5082594
GAGTACT70400.027.28927812-13
CATGGGG46450.026.507654
TAGTACG900.00954177326.4365084
GTACATG114400.026.3271771
ACATGGG111400.026.056813
TACATGG115650.025.8707472
AGTACTT71650.024.8208812-13
GGGGTGA17950.024.5218877
Produced by FastQC (version 0.11.5)