FastQCFastQC Report
Fri 10 Feb 2017
ERR1147744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816103
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67290.37051863247844424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42750.23539413788755373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30990.17064010135988983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.060.384221
GTATCAA54550.056.104331
TCAACGC73050.041.6903044
ATCAACG73700.041.1611983
CAACGCA75250.040.5504955
AACGCAG76450.040.0696036
TATCAAC81300.037.6070982
ACGCAGA90900.033.569037
CGCAGAG93200.032.7379078
GCAGAGT102250.030.0729949
GTACATG94650.029.3781361
TACATGG98850.028.2223662
ACATGGG99250.027.688333
CATGGGT22500.027.229524
GAGTACT38650.026.31383312-13
AGAGTAC95050.025.84255210-11
CATGGGG52500.024.2459834
CAGAGTA99100.024.09624510-11
TATACCG2502.0168955E-823.7927885
GTACTTT45150.022.45970714-15
Produced by FastQC (version 0.11.5)