FastQCFastQC Report
Fri 10 Feb 2017
ERR1147743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912178
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81980.8987280991210048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55090.6039391434566499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.4461848455016455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.1482166857784336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.047.9431571
GTATCAA65050.046.6568831
AACGCAG79650.037.7767146
TATCAAC81350.037.0603752
ATCAACG83200.036.2363173
TCAACGC83250.036.1431244
CAACGCA84050.035.728365
ACGCAGA91250.032.8440787
CGCAGAG93350.032.103468
GCAGAGT99350.030.22459
GTACATG81100.027.4437581
GAGTACT46250.027.19156612-13
TACATGG82600.026.9966982
ACATGGG82250.026.8223863
CATGGGT12750.026.584234
CAGAGTA98450.024.9140210-11
CGTTACC1200.001292116624.7769682
CATGGGC20250.024.666854
AGAGTAC97050.024.35438710-11
CATGGGG42450.024.0940694
Produced by FastQC (version 0.11.5)