FastQCFastQC Report
Fri 10 Feb 2017
ERR1147742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986077
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119181.2086277237984457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.7560261521159098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57990.5880879485070638No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25180.2553553120091028No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22100.2241204287291966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21590.2189484188354459No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.1288945995089633No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10890.1104376230253824No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.10039783911398399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144550.032.8317071
ATGGGGG24250.030.6689515
AACGCAG156600.030.052836
CATGGGG59450.029.8239464
GTATTCG1004.4181012E-429.7730451
TATCAAC168800.028.0922532
ATCAACG171700.027.5831243
TAGATCG1306.378988E-527.460515
CAACGCA173350.027.2176135
TCAACGC173550.027.1862474
GAGTACT71600.027.0011912-13
CGCAGAG174050.026.9317638
ACGCAGA174300.026.8958637
GCAGAGT182350.025.7711589
AGTACTT74400.025.58524912-13
GGTATCA104150.025.0419451
GTAAGTA8700.024.6197662
TGGGGGG23150.024.4159326
CATGGGT16950.024.220334
ACATGGG147700.023.9682943
Produced by FastQC (version 0.11.5)