FastQCFastQC Report
Fri 10 Feb 2017
ERR1147741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879312
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132820.7067480013962557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89110.4741628851409452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64250.3418804328392518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.12387512025677481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103300.050.6100431
GGTATCA59850.049.7448771
AACGCAG134300.038.7124026
TCAACGC136950.038.1370544
ATCAACG137850.037.9312173
CAACGCA139600.037.37055
TATCAAC144450.036.363812
ACGCAGA156500.033.182927
CGCAGAG158250.032.735568
TATCACG1152.768846E-531.0369932
GCAGAGT171100.030.346579
GTACATG143400.027.7791841
TACATGG146000.027.3398362
ACATGGG142750.027.2114563
GAGTACT75950.026.27302712-13
CATGGGG76150.025.7785634
AGAGTAC163650.025.67683610-11
AGTACTT77150.025.63309712-13
GTATACG1401.05090294E-425.5191211
CAGAGTA166700.024.51130910-11
Produced by FastQC (version 0.11.5)