FastQCFastQC Report
Fri 10 Feb 2017
ERR1147740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650007
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67510.4091497793645724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44550.269998854550314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32970.19981733410827954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.063.7761231
GTATCAA52100.053.2406161
TCAACGC66800.040.9625174
CAACGCA67850.040.1532675
ATCAACG68700.039.6564643
AACGCAG70750.038.5074046
TATCAAC76450.035.9475862
ACGCAGA83750.032.3880847
CGCAGAG86550.031.4090218
GCAGAGT92050.029.5969499
GTACATG81350.026.2682881
TACATGG83750.025.4274882
CAGAGTA91200.024.91498410-11
AGAGTAC87100.024.65364510-11
ACATGGG84200.024.443833
GAGTACT41450.023.96536412-13
AGTACTT43900.021.54391712-13
GTACTTT46550.021.27583714-15
CATGGGG40550.020.6839524
CATGGGT20400.020.4114174
Produced by FastQC (version 0.11.5)