FastQCFastQC Report
Fri 10 Feb 2017
ERR1147733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4060264
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330490.8139618507565026No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA279280.6878370470491574No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT246790.6078176197409824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179230.4414244985055159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177970.43832125201711025No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG131140.32298392419803246No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG112110.27611505064695296No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG90040.22175897921908527No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT88560.2181138960422278No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC85620.21087298756928122No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC82740.20377985273863966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82530.20326264499057203No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA80490.19823834115220096No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC77540.19097280373887018No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC70840.1744714136814749No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT63930.157452815876012No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59160.14570481131276194No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG42980.10585518577117152No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC40650.10011664266165944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3500.049.2844922
ATGGGGG81800.035.5570035
TATAACG5300.032.546362
CATGGGG133350.032.3381084
TGGGGGG67300.031.7272876
GGGTTAG10750.028.7911321
CGCATAG5400.028.657841
TACAACG6300.027.3802722
GTTAAGA26400.025.2338624
TAACGCA8250.024.5128944
GGTTAAG25100.024.1710823
AGTACTC32750.023.7919275
CATGGGT45750.023.1418744
ACAACGC8450.022.52493
TCACGCA8250.022.349994
GGTTAGA12850.022.2186892
CACTATA10550.022.0027031
ATCACGC8250.021.6290253
GTGGTTA21750.021.6188071
TTAGAGC14600.021.1845914
Produced by FastQC (version 0.11.5)