FastQCFastQC Report
Fri 10 Feb 2017
ERR1147723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108495
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271270.872673110299357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA201060.6468081820945506No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT178140.5730747516080933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156620.5038451083241247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136770.4399878397745533No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG104350.33569299612835146No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG84230.27096714004687156No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT76590.24638932988471912No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70360.2263474768336446No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC68000.21875537840659226No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65600.21103460034518312No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA61370.19742672901194952No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59720.19211869409473073No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC57020.18343281877564546No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT55430.17831780330996191No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC52080.16754088393257832No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.11520044265794219No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC32830.10561380989835918No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG32130.10336191629711484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3300.045.0627442
TATAACG3300.039.6552122
GGGTTAG9100.035.9858861
CGCATAG4850.035.601411
ATGGGGG64600.031.951815
GTGGTTA18900.031.8178271
CATGGGG103800.031.4037674
TACAACG4550.030.0682372
GTTAAGA26800.029.9638424
ATCACGC5650.029.478213
GGTTAAG25050.029.2071343
TGGGGGG55150.028.3635046
GGTTAGA11050.027.9919132
GTTAGAG11950.027.3770313
AAACGCA7750.026.8636425
TTAGAGC12150.026.4372314
GGTTTCA13350.026.3136861
TCACGCA5900.026.213194
TAACGCA5950.025.992914
CATGGGT36250.025.5985364
Produced by FastQC (version 0.11.5)