FastQCFastQC Report
Fri 10 Feb 2017
ERR1147714.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147714.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614598
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211300.8081548291553806No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA155700.5955026355868092No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT140150.5360288656229371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125520.4800738010202716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112840.4315768619114678No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78150.29889872171553716No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG64480.2466153496636959No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT60010.22951903122392045No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55290.21146654284903452No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG52280.1999542568303043No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50340.19253437813384697No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC46240.17685319119803503No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC45410.17367870701346821No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44730.1710779247899677No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC41700.15948914517642865No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT41500.15872420922834027No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.12522001470206892No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC31220.11940650149659718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2600.057.1954232
GGGTTAG10700.046.1676941
GGTTAGA12350.039.9765852
GTTAGAG12550.039.813483
TTAGAGC13500.038.333644
ATGGGGG58200.035.1584785
TAGAGCA14400.034.6985555
CATGGGG89150.034.1619954
CGCATAG3750.033.3297351
TCACGCA4600.032.3278474
ATCACGC5500.030.2823753
GGTTTCA10050.030.2028541
TGGGGGG51100.029.9133646
CATGGGT27900.029.635024
TACAACG2751.8189894E-1228.1193492
GTGGTTA16100.027.725491
GTATCAC7200.027.2788011
TATAAGT6300.026.4369964
GTTAAGA23450.025.6196464
AGTACTC20000.025.2803755
Produced by FastQC (version 0.11.5)