FastQCFastQC Report
Fri 10 Feb 2017
ERR1147711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3694027
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327930.8877303820464767No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA196450.5318044508066672No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT180810.4894658322746423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179110.48486380852116134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178540.4833207770273471No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG96590.26147616138160334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77040.20855288821657234No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG75460.20427571319863122No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG66190.17918114837817914No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC63420.17168255673280136No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT62970.170464373974527No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA59010.15974436570171252No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC56600.15322032026295423No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC53730.1454510213379599No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC52610.1424190998062548No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT46090.12476898517525725No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA40000.10828291184661076No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.10552169759452219No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA38010.10289583698224188No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.10230028096708552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3650.042.3667222
TATCACG4150.040.1286282
TACAACG6400.033.4554522
CATGGGG105700.031.0042324
ATGGGGG56550.030.816225
TAACGCA5800.030.7636364
CGCATAG5550.030.0321081
ACAACGC7900.028.6088833
TGGGGGG47850.026.5985896
TCACGCA7650.023.3240624
GTATAAC7950.023.2121471
AGTACTT256250.022.06048212-13
CATGGGT40600.021.6810384
AACGCAG656850.021.250726
ATCACGC7850.021.2144993
ATGGGTG25700.021.0597235
GAGTACT244750.020.84929312-13
GTATCAA678550.020.3704741
GGTTTCA17200.020.0733721
CACTATA9800.020.0451181
Produced by FastQC (version 0.11.5)