FastQCFastQC Report
Fri 10 Feb 2017
ERR1147709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290996
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293840.8928603984933438No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA192950.5862966712812777No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT176600.5366156628570804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167110.5077794078145339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160500.4876943028797361No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG91130.27690705184691805No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG76100.2312369872221054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72870.22142232928876243No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT62490.18988172577541876No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG61820.18784586793785227No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC59530.1808874881646772No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59200.17988475221483102No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA57000.1731998458825231No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC54130.1644790817126487No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC53650.16302055669469062No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50510.15347937220221478No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48680.14791874557124957No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT45300.13764829856979466No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT35900.10908551696811543No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA35520.10793085132889861No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33600.10209675125706626No Hit
GGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAG33110.10060784030123404No Hit
GGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAG33020.10033436686036688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG18600.047.6911051
GGTTAGA19300.045.3031042
GTTAGAG20250.043.4714973
TATAACG3150.043.429542
TAGAGCA21800.042.017695
TTAGAGC21900.041.5542344
TACAACG4300.037.347652
ATGGGGG87300.036.7914965
CATGGGG126200.035.5840644
GTTAAGA30250.033.8197984
CTATAGC8300.033.6812133
TGGGGGG74000.033.5953756
GTGGTTA20150.033.0907821
GGTTAAG28750.032.0672573
TTAGCGT1301.7420389E-632.027474
CACTATA8850.031.6168541
ACAACGC5400.030.8412673
ACTATAG9650.029.5857012
GTACATT15500.029.1907981
GTATAAC7150.026.6445241
Produced by FastQC (version 0.11.5)