FastQCFastQC Report
Fri 10 Feb 2017
ERR1147669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583937
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA538321.5020353315362407No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT445421.2428231857870269No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302260.8433741999371083No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT241840.6747886472334753No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG226990.6333537671002587No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG222960.622109149798113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173520.4841602963444949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173470.484020784963575No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC168000.4687582398909356No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA160780.4486127964860989No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC160750.44852908965754695No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG153810.42916490998586193No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC143900.4015137542875335No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC143310.39986751999267844No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT114820.3203739351445073No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG89420.24950215363718728No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA84120.2347139472596756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83900.23410009718362795No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT80050.223357720852794No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT79590.22207421614833073No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC78000.21763775423507725No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA75740.21133183981749676No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC71700.20005932023916712No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC71490.1994733724393035No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG70720.19732489717313667No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA64400.17969065862485864No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG63080.17600755816857272No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC57300.1598800425342298No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC55870.15589001703992006No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54630.15243013479310602No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT53810.1501421481460193No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG53620.1496120048985236No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC52560.14665436362302126No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG51470.143613015518967No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG47890.13362400064510063No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC47510.13256371415010923No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC46680.13024782522683853No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG45480.12689955208476042No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA44380.12383030170452214No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC40320.11250197757382455No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT38800.10826083159385894No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG38280.10680991323229175No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38200.10658669502281988No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA36080.10067141247181521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4700.043.0315932
TACAACG4750.038.821692
AGTACTC42950.036.7019425
AAGTACT51650.031.6715074
TCACGCA6500.031.115154
GGGTTAG9800.030.982341
GTTAAGA21200.030.864784
AAAGTAC51450.030.1759873
GTGGTTA17000.028.7167321
ATGGGGG52350.028.6345395
GGTTAAG21850.027.768673
ATCACGC7850.027.2796753
CATGGGG100600.027.199834
GTTAGAG12150.026.437713
CGCATAG5300.025.835811
AGAGCGC8300.025.7981388
AAAAGTA59550.025.372212
TATAACG3051.0913936E-1125.3541982
GGTTAGA11800.025.2054442
TGGGGGG43950.025.1709616
Produced by FastQC (version 0.11.5)