FastQCFastQC Report
Fri 10 Feb 2017
ERR1147657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594325
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371452.329826101955373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232171.456227557116648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190471.1946748624025842No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84710.5313220328352124No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA48720.30558386778103586No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA48110.30175779718689727No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.2979317265927587No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46670.29272576168597997No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA34260.21488717795932447No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA32010.20077462248914119No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.19958289558277015No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA30250.18973546798802002No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28290.17744186411177143No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28240.17712825176798958No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA27880.17487024289276026No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG27870.17480752042400388No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22850.14332084110830604No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC20100.1260721622003042No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19190.12036441754347452No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19020.11929813557461622No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA18090.11346494598027379No Hit
GGTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAA16390.10280212629169083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1155.456968E-1251.720242
CGCATAG2250.047.6289331
TTAGAGC4950.038.4506034
GTTAGAG4950.038.4506033
GGGTTAG4750.037.6017881
AACGCAG374850.036.428866
GCGTATC1209.2473783E-734.729431
GTACATT4600.033.6508751
GGTTAGA5350.033.3523062
ATGGGGG36300.033.261965
GGTTTCA6500.032.9738771
GTATCAA414950.032.6412281
TAGTACT3500.032.288215
TAGAGCA5800.031.7901175
ACGCAGA432450.031.5079637
CGCAGAG433100.031.4744078
TAATACG953.2939267E-431.3043564
TAGGACG953.2939267E-431.3043564
CATGGGG69250.031.2636744
GCATAGT3500.030.588832
Produced by FastQC (version 0.11.5)