FastQCFastQC Report
Fri 10 Feb 2017
ERR1147642.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147642.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870599
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267980.9335333844957099No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA174460.6077477209460465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159580.555911849756793No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT155820.5428135382197234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142500.4964120728809562No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG91590.31906232810643353No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT72070.2510625831054773No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG69500.24210974782615055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66590.23197249075889737No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG62210.21671435125560903No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA58760.20469595370164903No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC57710.20103818053305253No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC56000.19508123565848104No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT49500.17243787794812163No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC48950.17052190152647584No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46710.16271865210013658No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC44270.15421868397501706No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC35020.12199544415642867No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31320.10910614822899332No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31190.10865328107478613No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA30490.10621476562905513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3150.060.420962
TATAACG3800.045.3902552
TACAACG4250.040.584232
CGCATAG3300.039.680291
ATGGGGG64950.034.1568575
TCACGCA5750.034.134564
TAACGCA5900.033.266734
CATGGGG105800.032.6054764
GGTTTCA12150.031.8422031
GGGTTAG10450.031.326541
ATTAACG3850.030.8970813
GTGGTTA21650.030.7911681
GGTTAAG25000.030.2142563
GTTAAGA25650.029.9123524
ATCACGC6450.029.507913
TTAACGC3700.028.9346984
TGGGGGG55850.028.856856
CATGGGT46800.028.8488314
ACGCATA2154.0254236E-927.6839221
GTATACG2154.0254236E-927.6839221
Produced by FastQC (version 0.11.5)