FastQCFastQC Report
Fri 10 Feb 2017
ERR1147639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3756890
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354320.9431205065892267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192020.5111142460918473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190750.507733790449015No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA129530.3447798578079209No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT122310.32556183438961483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84890.22595817285041617No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78360.2085767749388457No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64190.17085940764834745No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50830.135298078996191No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG47520.12648760011605345No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47090.12534303639446456No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC45430.12092448807391219No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.11996624867909361No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42930.11427004783211646No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42160.11222048023764336No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA38680.10295749942106369No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC38210.1017064646556061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3250.043.9227942
ATGGGGG80150.034.4331365
GGGTTAG10950.034.24621
CATGGGG127600.033.3752444
TATAACG3350.031.9587542
TACAACG4100.031.915452
TGGGGGG65650.031.0711926
CGCATAG4300.030.453711
GTTAGAG13550.029.8491333
TTAACGC5250.028.3232334
GGTTAGA13300.027.7269552
TAACGCA6700.027.5200375
CATGGGT53950.027.451754
GTTAAGA29350.026.7502564
AACGCAG582300.026.717116
ATTAACG5800.026.6629053
GTATCAA584000.026.1840741
TTAGAGC15450.026.1783664
TAGAGCA14800.026.1224385
GTGGTTA21500.026.0240781
Produced by FastQC (version 0.11.5)