FastQCFastQC Report
Fri 10 Feb 2017
ERR1147632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250714
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288650.8879587684428712No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA178340.5486179343984121No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT168200.5174247872928839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162440.49970560313826434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130170.4004351044109079No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG99530.30617888869952875No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG78780.24234675828141142No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT69370.2133992716677013No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC67550.20780050167440137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67300.20703143986213493No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA63440.19515712548074055No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC62620.1926346027365065No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54920.1689474989186991No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG54110.16645573864695573No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT52430.16128764326852502No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50260.15461218673805202No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC50200.15442761190310805No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC46770.14387608383881204No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33920.10434630668831524No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.10111624707679606No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG32590.1002548978470576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3150.045.315742
TATAACG3300.039.6512762
GTGGTTA23600.038.5870821
GTTAAGA32600.037.7660184
ATGGGGG85900.035.3814935
GGTTAAG33500.035.153523
GGGTTAG13900.035.112481
CATGGGG125950.033.5280724
CGCATAG3450.032.77911
TGGGGGG71450.032.629136
ATATGTA21050.031.385811
GTTAGAG15600.031.2635063
TACAACG4700.030.3711872
GCGGTTA8650.030.2760411
GTACATT14250.029.655551
TATGTAA22600.029.4752832
AGAGCGC9750.029.2791398
TAGAGCA18050.028.9970555
TTAGAGC17050.028.953574
GGTTAGA16600.028.663572
Produced by FastQC (version 0.11.5)