FastQCFastQC Report
Fri 10 Feb 2017
ERR1147629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3986723
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345770.8673037981319495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197940.4964980009897853No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA192610.48312862468749396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179820.45104713821351516No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT170000.4264153792475675No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG109210.27393425628015794No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG83850.21032311499946196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82060.2058332118885611No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT75170.18855084740023323No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG71710.1798720402696651No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA71080.17829179504068882No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC69850.17520655435554464No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC64540.16188734456845885No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61890.15524028130371736No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT60820.15255637274021797No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC57620.14452973030732258No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC49420.12396145907302815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4400.044.6093642
TATCACG4400.040.5539672
TACAACG6800.037.6118132
ACAACGC7500.034.1013763
TAACGCA6650.033.0936624
GGGTTAG19550.031.968981
ATGGGGG76200.031.9251585
GTGGTTA24250.031.9093441
GTTAGAG21500.029.6012523
GGTTAGA20850.029.3829842
CGCATAG4900.029.1542171
CATGGGG130550.029.0219964
GGTTAAG28850.028.2448643
GTTAAGA29100.028.002214
CATGGGT56750.027.7744054
TGGGGGG66350.027.6077466
ATATGTA25100.026.560151
TTAGAGC23650.026.407234
TCACGCA7600.025.0438524
TAGAGCA25700.024.9951275
Produced by FastQC (version 0.11.5)