FastQCFastQC Report
Fri 10 Feb 2017
ERR1147602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119385
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109900.981789107411659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60210.5378846420132483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56000.5002747044135842No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.27622310465121475No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28310.2529067300347959No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23980.2142247752113884No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.18117091081263373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20200.18045623266347144No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17530.15660384943518094No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.12873140161785265No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12520.1118471303438942No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12460.1113111217320225No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11820.10559369653872439No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11500.10273498394207532No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG902.3878536E-433.0675661
TAACGCA1203.705945E-529.7382055
ATTAACG1306.395362E-527.4506533
GTATCAA191550.026.6922861
AACGCAG187800.026.3776426
GTAGCAC1701.4030236E-524.4902863
GCCTTAT2001.8200153E-623.8086451
AGTCCGT1250.001638552823.7873766
ACGCAGA210050.023.5835347
CGCAGAG210100.023.577928
TATCAAC215650.023.5257952
ATCAACG216650.023.1975863
TCAACGC219600.022.9672134
GCAGAGT216600.022.842919
CAACGCA221200.022.8010835
GCACCGT1903.319092E-521.9094266
CATGGGG27750.021.647274
TATTGGA8050.021.4262852
CATAAGT1400.003162874121.2415754
GGGTTAG1450.003859184420.5246941
Produced by FastQC (version 0.11.5)