FastQCFastQC Report
Fri 10 Feb 2017
ERR1147600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4839638
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382280.7898937895768238No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA332900.6878613648376181No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT303040.6261625352970615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219690.4539389103069279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215750.44579780553834814No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG153600.31737910975986217No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG134100.2770868399661297No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT130420.26948296546146633No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA114080.23572010964456433No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC111910.23123630321110794No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC105310.21759891958861385No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG104490.21590457798703125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95240.19679157821308121No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC91380.18881577506416802No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC91150.18834053290762656No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC89100.18410467890367005No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT82650.17077723581805085No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81100.1675745169370106No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG57470.1187485510279901No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG55210.11407878027240881No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51190.10577237388416241No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC50460.10426399660470474No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT50330.1039953814727465No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT48590.10040007124499808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4350.043.7498442
GGGTTAG20950.040.6230961
GTTAGAG22150.037.8583073
TATAACG4100.037.7142332
ATGGGGG129150.036.885355
GGTTAGA23950.035.757962
TACAACG5550.035.361992
TAGAGCA25900.035.132375
CATGGGG188900.034.3485644
TGGGGGG110850.033.1547786
TTAGAGC25950.032.543694
GTACATT24500.032.307741
TAACGCA6950.031.6615894
ATATGTA33250.030.4283431
TACATTG26250.029.9061432
CGCATAG5050.029.4625051
TATGTAA35300.028.8095972
TCACGCA7400.025.7178134
ATTGTAG29650.025.6744445
GTGGTTA23200.025.6526971
Produced by FastQC (version 0.11.5)