FastQCFastQC Report
Fri 10 Feb 2017
ERR1147598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501544
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194890.7790788409078553No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT155960.6234549542202735No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA154420.6172987562881165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117330.469030326870125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87920.35146293649042354No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG84090.3361523922825263No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70760.2828653023892444No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT65170.26051910340173906No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC60850.24324976894270098No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA46830.18720438257332273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46280.18500574045469517No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT46180.18460598734221745No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG45580.18220746866735105No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC45040.18004880185997127No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44500.17789013505259152No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC42610.17033480122676234No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27050.10813321692522697No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG26760.10697393289904156No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.10649422916406827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3100.040.2947542
CGCATAG3850.040.200691
TACAACG4000.035.6896362
TATCACG2300.033.6206742
GGGTTAG6000.032.740371
GTTAGAG6950.028.2435993
CATGGGG66500.027.4604474
AGTACTC19950.026.8343125
GGTTAGA6900.026.7241252
ATGGGGG33350.026.5754935
TAACGCA4750.026.2976264
CATGGGT28000.026.1299134
GTGGTTA9950.025.1273751
GTTAAGA12600.024.0763444
ACAACGC6450.023.9775353
GGTTAAG12200.023.8906063
AAGTACT25350.023.6992344
TAGTACT6900.023.275855
GTATAAC7200.023.1497571
ATGGGTA15600.022.4966725
Produced by FastQC (version 0.11.5)