FastQCFastQC Report
Fri 10 Feb 2017
ERR1147596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370367
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351501.0429131308252186No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA253250.7514018503029493No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT228960.6793325474644156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196610.5833489349972867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184100.5462313154620847No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG139170.412922390944369No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG104860.31112338804646494No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT94950.28172006193984217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88840.2635914723826812No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC88050.26124751399476676No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG82250.24403870557716711No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA78420.23267495794968324No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC75050.22267604685187103No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC72330.2146057091112036No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70830.21015515521010025No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC66180.19635843811667986No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63820.18935623331227727No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46830.13894629279244664No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43880.1301935367869434No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA41050.12179682509352839No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG40750.12090671431330773No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC40660.12063968107924151No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39730.11788033766055744No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC37730.11194626579241963No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT37020.10983967027923072No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA36020.10687263434516181No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC34450.10221438792867364No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA33890.10055284780559506No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG33780.10022647385284748No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA33780.10022647385284748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3150.047.208012
TACAACG5100.038.488422
CGCATAG4350.038.3146131
GGGTTAG12300.038.231181
ATGGGGG69050.037.3862885
TATAACG3050.037.054422
CTATAGC10600.034.7914163
GTTAGAG14250.034.6457143
GGTTAAG25000.034.499623
GTTAAGA26350.034.0865174
TGGGGGG59300.033.6981776
CATGGGG119450.033.3637584
CACTATA11400.032.8951151
GGTTAGA14500.032.4074862
GTGGTTA19750.032.248711
GGTTTCA13050.031.0165921
ACAACGC6550.030.8762053
TAGAGCA17550.030.503645
TTAGAGC16750.029.8298284
AGAGCGC10250.029.5915818
Produced by FastQC (version 0.11.5)