FastQCFastQC Report
Fri 10 Feb 2017
ERR1147589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4319509
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243170.5629575028087683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142410.3296902495167854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111680.2585479044030236No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT43440.10056698573842537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA175250.054.8955881
GGTATCA91450.052.9901121
AACGCAG219800.043.1402666
TCAACGC228600.041.558124
ATCAACG230200.041.2707063
CAACGCA234650.040.435455
TATCAAC251200.038.152082
ACGCAGA256900.036.956097
CGCAGAG264300.035.943468
GCAGAGT284250.033.5463459
GTACATG250100.031.9204651
TACATGG257700.030.9799272
ACATGGG257150.030.4215643
CATGGGG123150.028.0652524
CATGGGT80000.028.033584
AGAGTAC271950.027.28749310-11
GAGTACT134150.026.00695412-13
CAGAGTA279150.025.79522510-11
ATGGGGG51200.024.5151585
AGTACTT140600.024.43311312-13
Produced by FastQC (version 0.11.5)