FastQCFastQC Report
Fri 10 Feb 2017
ERR1147588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3582442
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221030.6169813775072981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136680.38152746087724515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109360.3052666309740674No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38480.10741276481238218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87650.055.959521
GTATCAA159650.052.9441681
AACGCAG200600.041.9283646
TCAACGC212000.039.898194
ATCAACG212650.039.6648983
CAACGCA215550.039.1307075
TATCAAC225900.037.6548922
ACGCAGA238400.035.204497
CGCAGAG242850.034.5584378
GTACATG229250.032.7679061
GCAGAGT262250.032.0926939
TACATGG235300.031.5496272
ACATGGG232700.031.2626363
CATGGGT62650.029.3377534
CATGGGG122700.029.135294
ATGGGGG57600.027.5726325
AGAGTAC252950.026.45349310-11
GAGTACT124850.025.94027112-13
CAGAGTA259300.025.64510510-11
AGTACTT129800.024.35531812-13
Produced by FastQC (version 0.11.5)