FastQCFastQC Report
Fri 10 Feb 2017
ERR1147587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912579
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134440.7029252125010261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80870.4228322071924872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63010.3294504436156624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22300.11659649091619222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106550.053.190291
GGTATCA58100.051.334631
AACGCAG133000.042.2220956
ATCAACG138150.040.734253
TCAACGC140400.040.0814554
CAACGCA143300.039.187295
TATCAAC145050.039.0436132
ACGCAGA156450.035.8935057
CGCAGAG159000.035.3178528
GCAGAGT168300.033.5076269
GTACATG148650.030.757081
TACATGG150400.030.258292
ACATGGG149900.030.0012683
CATGGGG72500.029.1279414
ATGGGGG31500.028.1381245
CATGGGT45500.027.5861134
TATAACG1107.762121E-427.0400892
GTATCGA1556.846367E-626.8649339
GAGTACT78550.026.6951512-13
AGAGTAC165100.026.3384210-11
Produced by FastQC (version 0.11.5)