FastQCFastQC Report
Fri 10 Feb 2017
ERR1147586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827781
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135500.4791743066383147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80150.2834377909746193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64720.22887203782754037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65300.050.6821061
GTATCAA118850.050.484071
AACGCAG146850.040.4285746
TCAACGC152650.038.8535044
ATCAACG155250.038.16453
CAACGCA156800.037.74935
TATCAAC166800.035.7001382
ACGCAGA170900.034.7032057
CGCAGAG174750.033.9386448
GCAGAGT187000.031.7471969
GTACATG170900.030.1974351
TACATGG176150.028.975872
TATAACG2900.028.7185082
ACATGGG175050.028.682183
GTATAGG6050.027.5483551
CATGGGT49750.027.3825724
AGAGTAC181800.026.27472310-11
CATGGGG89500.025.9222934
TAACGCA4300.024.9020964
CAGAGTA187300.024.80445910-11
Produced by FastQC (version 0.11.5)