FastQCFastQC Report
Fri 10 Feb 2017
ERR1147585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151068
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174110.5525428204024794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101650.3225890396525876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79030.25080385443919334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120400.060.519881
GGTATCA59300.059.5309941
TCAACGC163400.044.4818764
AACGCAG164650.044.0712286
ATCAACG166050.043.738943
CAACGCA168050.043.2503625
TATCAAC179850.040.779712
ACGCAGA193350.037.4679687
CGCAGAG198800.036.5006458
GCAGAGT213800.034.134539
GTACATG193250.032.006571
TACATGG198700.031.0436232
ACATGGG198950.030.4664423
CATGGGT55850.029.7128944
CATGGGG104850.028.8176944
AGAGTAC204350.028.30557810-11
GAGTACT93300.026.64720512-13
ATGGGGG52800.026.5849085
AGTACTT98450.026.15948712-13
CAGAGTA210800.025.77478210-11
Produced by FastQC (version 0.11.5)