FastQCFastQC Report
Fri 10 Feb 2017
ERR1147584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301586
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313560.9497253744109649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181470.5496449282254044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144760.43845594208359256No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53300.1614375636436549No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43180.13078562848279585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA214000.055.2581561
GGTATCA113300.053.4993861
AACGCAG274950.042.689656
ATCAACG285900.041.1605453
TCAACGC287650.040.9082764
TATCAAC290700.040.7060282
CAACGCA294650.039.7950865
ACGCAGA319450.036.7592967
CGCAGAG323400.036.310328
GCAGAGT345800.034.044259
GTACATG300850.030.5582311
TACATGG306250.029.9361212
ACATGGG304750.029.8101623
CATGGGT59750.028.3763284
CATGGGG131750.027.6795544
ATGGGAG46700.027.3886645
AGAGTAC337800.027.33096910-11
GAGTACT162550.026.51394512-13
ATGGGGG58050.025.9279145
CAGAGTA343800.025.89368210-11
Produced by FastQC (version 0.11.5)