FastQCFastQC Report
Fri 10 Feb 2017
ERR1147583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569834
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162980.6342043882990107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104090.40504561773250725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78420.30515589722915953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27400.10662167283956862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118950.053.194771
GGTATCA65050.052.1588131
AACGCAG149150.042.073456
TCAACGC156700.040.198134
ATCAACG157050.040.1850783
CAACGCA160050.039.3567475
TATCAAC167050.037.9582822
ACGCAGA175450.035.7320337
CGCAGAG179800.034.7683038
GCAGAGT192450.032.606569
GTACATG163650.029.244231
TACATGG166800.028.3865622
ACATGGG164650.028.0341473
AGAGTAC185400.026.61197510-11
CATGGGG92100.026.6082614
GAGTACT90900.026.3700912-13
CAGAGTA190450.025.40662810-11
CATGGGT41300.024.9158064
AGTACTT95150.024.66088712-13
ATGGGGG45400.023.5828115
Produced by FastQC (version 0.11.5)