FastQCFastQC Report
Fri 10 Feb 2017
ERR1147582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611160
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190400.7291778366702921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117100.4484596884143445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93630.35857626495503914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34020.13028692228741248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29500.11297660809755052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74350.052.207281
GTATCAA129700.050.078261
AACGCAG160500.040.2495886
TCAACGC172400.037.436844
ATCAACG173950.037.1388743
CAACGCA174500.036.9522175
TATCAAC178900.036.1777762
ACGCAGA190150.034.0673377
GTACATG175800.033.6952481
CGCAGAG194250.033.28648
CATGGGG103200.032.6244664
ATGGGGG52150.032.508525
TACATGG181950.032.3346062
ACATGGG179400.032.3299833
GCAGAGT209800.030.9326869
TGGGGGG45550.026.9020586
AGAGTAC201950.026.73013310-11
GTAAGTA22950.026.6979452
CAGAGTA205500.025.2118510-11
GAGTACT109300.025.03433412-13
Produced by FastQC (version 0.11.5)