FastQCFastQC Report
Fri 10 Feb 2017
ERR1147581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288853
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168350.5118805857239591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100780.30642901947882745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77840.236678258347211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.059.2896081
GTATCAA122100.055.036061
TCAACGC161550.041.3857654
AACGCAG162550.041.277546
ATCAACG162400.041.2070353
CAACGCA165150.040.4836245
TATCAAC175750.038.41542
ACGCAGA192550.034.8447577
CGCAGAG196400.034.1919828
GCAGAGT212500.031.657429
GTACATG199050.031.517191
TACATGG203300.030.8102952
ACATGGG203200.030.0936093
CATGGGG110250.028.4870194
CATGGGT50700.028.274884
GAGTACT93000.025.806712-13
AGAGTAC204700.025.8028110-11
CAGAGTA210600.024.95284310-11
ATGGGGG60550.024.85415
TGGGGGG49950.022.030686
Produced by FastQC (version 0.11.5)