FastQCFastQC Report
Fri 10 Feb 2017
ERR1147580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3606106
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200290.5554190586743706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117690.3263631185550286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96600.2678789808175356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144950.058.8062741
GGTATCA72250.056.353011
TCAACGC200150.042.648724
AACGCAG200650.042.51286
ATCAACG201600.042.22573
CAACGCA205300.041.433995
TATCAAC215500.039.6677252
ACGCAGA233550.036.4965637
CGCAGAG239900.035.5538338
GCAGAGT257750.033.160859
GTACATG240700.030.9124051
TACATGG245500.030.1194762
ACATGGG246050.029.8345583
CATGGGG129650.028.1712154
CATGGGT65400.027.9235324
ATGGGGG65050.027.7994375
AGAGTAC247850.027.53793510-11
GAGTACT107400.025.08773612-13
CAGAGTA254150.024.93623510-11
AGTACTT113650.024.83329412-13
Produced by FastQC (version 0.11.5)