FastQCFastQC Report
Fri 10 Feb 2017
ERR1147579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3416314
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177090.5183657005767034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104220.30506563506750256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84740.24804511529092468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137400.057.756911
GGTATCA70050.052.3519821
AACGCAG177100.044.3363346
TCAACGC180700.043.518884
ATCAACG183850.042.8398363
CAACGCA186000.042.15095
TATCAAC199700.039.7375642
ACGCAGA207100.037.9133267
CGCAGAG212850.036.8885848
GCAGAGT229050.034.3834469
GTACATG202650.030.8463081
TACATGG207900.029.9581972
ACATGGG207750.029.2639753
AGAGTAC220300.028.08086810-11
CATGGGT56900.026.8673764
CATGGGG105450.026.2871634
CAGAGTA227150.025.44027110-11
AGTACTT112850.025.01072712-13
GAGTACT108250.024.69980212-13
ATGGGTA28450.023.417485
Produced by FastQC (version 0.11.5)