FastQCFastQC Report
Fri 10 Feb 2017
ERR1147578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4202149
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224450.5341314646386884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125560.29879949521066484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102640.24425597474054347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88950.057.0209471
GTATCAA173200.056.1279341
AACGCAG227100.042.3266076
TCAACGC230300.041.8164634
ATCAACG233050.041.402063
CAACGCA236150.040.7297065
TATCAAC247550.039.409522
ACGCAGA266550.036.0613177
CGCAGAG274800.035.0215648
GCAGAGT293450.032.85669
GTACATG277050.030.769871
TACATGG283650.029.9688222
ACATGGG281150.029.7698233
CATGGGG145750.028.7723264
CATGGGT84300.027.236684
AGAGTAC284950.027.0004810-11
ATGGGGG75400.026.4279025
GAGTACT126400.025.83443612-13
CAGAGTA291150.025.33253310-11
AGTACTT132050.024.77410712-13
Produced by FastQC (version 0.11.5)