FastQCFastQC Report
Fri 10 Feb 2017
ERR1147577.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147577.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943982
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274060.9309160178289134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167390.5685836394380129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132030.4484742094211174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44310.1505104311099728No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38070.12931464934228537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121200.051.672051
GTATCAA210950.051.191791
AACGCAG259650.041.466126
ATCAACG273000.039.5275543
TCAACGC277100.038.9407164
TATCAAC283150.038.2996982
CAACGCA283400.037.99115
ACGCAGA302400.035.6434337
CGCAGAG307050.035.142398
GCAGAGT326250.033.1471759
CATGGGT99100.029.712194
GTACATG283400.029.5764051
TACATGG287800.029.102962
ACATGGG286500.028.7159273
ATGGGTG50150.027.1623735
CATGGGG125000.026.744194
GAGTACT152750.026.63655312-13
AGAGTAC320550.026.53648410-11
CAGAGTA324100.026.31005910-11
AGTACTT158400.025.0104912-13
Produced by FastQC (version 0.11.5)