FastQCFastQC Report
Fri 10 Feb 2017
ERR1147575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118336
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195620.6273217510877597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129090.41397078441835644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94540.3031745135867335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33820.10845527871274938No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31750.101817122978409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80150.057.775491
GTATCAA147800.053.0371
AACGCAG185450.042.30926
TCAACGC191650.040.8473474
ATCAACG193850.040.3230483
CAACGCA195300.040.0839425
TATCAAC203050.038.731042
ACGCAGA221100.035.4598247
CGCAGAG225900.034.679478
GCAGAGT240750.032.540369
GTACATG209200.029.9310321
TACATGG213450.029.1239932
ACATGGG211750.028.7954753
CATGGGG105800.026.7632034
CATGGGT65500.026.5190774
GAGTACT114450.026.45482412-13
CAGAGTA239100.026.06017710-11
AGAGTAC233500.025.86996810-11
AGTACTT120400.023.49237412-13
ATGGGTG36850.023.4070855
Produced by FastQC (version 0.11.5)