FastQCFastQC Report
Fri 10 Feb 2017
ERR1147574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625900
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155990.5940439468372748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104140.3965878365512776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79660.3033626566129708No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.10171750637876537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125200.049.5913431
GGTATCA71200.049.4121061
AACGCAG153850.040.2112276
TCAACGC163650.037.6941764
ATCAACG165050.037.230993
CAACGCA168750.036.5902255
TATCAAC176400.035.0715182
ACGCAGA179850.034.297577
CGCAGAG182550.033.8228768
TATCACG2400.032.221962
GCAGAGT197000.031.4325439
GTACATG167600.030.7943441
TACATGG170650.030.0483592
ACATGGG170000.029.8133283
TATAACG2401.2732926E-1129.7433452
CATGGGG85950.027.406964
CATGGGT46800.026.5651324
AGAGTAC190650.026.52021810-11
CAGAGTA194650.025.08902210-11
GAGTACT97600.024.95738612-13
Produced by FastQC (version 0.11.5)