FastQCFastQC Report
Fri 10 Feb 2017
ERR1147573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4171303
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255450.6123985718611187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145880.34972285638324524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115310.27643640368489175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA196700.055.7171021
GGTATCA102600.049.8988081
AACGCAG251750.043.165666
ATCAACG260550.041.7574163
TCAACGC262300.041.5206954
CAACGCA266050.040.8678975
TATCAAC273650.039.975812
ACGCAGA293450.037.0709157
CGCAGAG299700.036.317688
GCAGAGT321700.033.8894969
GTACATG297500.033.0569041
TACATGG301300.032.358682
ACATGGG301200.031.8361873
CATGGGT95700.030.2063924
CATGGGG142600.029.7402464
ATGGGGG69550.028.9915815
AGAGTAC310700.027.68093710-11
GAGTACT143850.025.63513412-13
CAGAGTA317950.025.23520710-11
AGTACTT151350.025.1311212-13
Produced by FastQC (version 0.11.5)