FastQCFastQC Report
Fri 10 Feb 2017
ERR1147572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5156633
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285490.5536364523129724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156010.30254237600387696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125610.2435891792183No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT54110.10493281177853843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA233500.054.7373121
GGTATCA125100.049.2517131
ATCAACG307700.041.2558063
TCAACGC308050.041.225844
AACGCAG311050.040.7509466
CAACGCA313800.040.4127775
TATCAAC326800.039.1911962
ACGCAGA358350.035.372077
CGCAGAG366500.034.585158
GCAGAGT393650.032.3206989
GTACATG361250.030.3707681
TACATGG370000.029.7598822
CATGGGT112300.029.4501744
ACATGGG368700.029.2834073
CATGGGG168050.028.0690364
GAGTACT161700.026.6690912-13
ATGGGGG81450.025.7791185
AGTACTT168250.025.75459312-13
AGAGTAC379000.025.7542910-11
CAGAGTA389250.024.22801410-11
Produced by FastQC (version 0.11.5)