FastQCFastQC Report
Fri 10 Feb 2017
ERR1147571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254788
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163580.5025826566891607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100070.3074547405238068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81030.24895630683165848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67550.055.2526741
GTATCAA127400.054.573841
AACGCAG169650.040.638756
TCAACGC171250.040.3632664
ATCAACG173300.039.955683
CAACGCA174900.039.350875
TATCAAC188950.036.8666842
ACGCAGA200200.034.3763737
CGCAGAG204300.033.685458
GTACATG204750.032.5034141
TACATGG209350.031.5977332
ACATGGG206300.031.4305063
GCAGAGT222150.031.1662149
CATGGGG109000.030.4521854
ATGGGGG56400.030.0591375
CATGGGT61800.028.2989064
AGAGTAC212550.027.0469410-11
GAGTACT98800.024.62315812-13
AGTACTT102950.024.58389312-13
CAGAGTA218700.024.4505310-11
Produced by FastQC (version 0.11.5)