FastQCFastQC Report
Fri 10 Feb 2017
ERR1147570.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147570.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945949
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81640.4195382304469438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50970.2619287555840364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38960.2002107968913882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38850.056.5419851
GTATCAA72300.054.0133631
ATCAACG96000.040.338683
TCAACGC95850.040.337674
AACGCAG96000.040.2116476
CAACGCA97350.039.715125
TATCAAC103900.037.5005532
ACGCAGA112550.034.244097
CGCAGAG115000.033.4628228
GTACATG122600.032.581251
TACATGG126350.031.6848562
ACATGGG124200.031.227553
GCAGAGT124750.030.9905249
CATGGGG60700.029.1043114
CATGGGT36250.028.3875334
ATGGGGG31550.026.3941155
AGAGTAC119400.025.72895410-11
GAGTACT52400.025.0284212-13
TATAACG1200.001290000224.7856712
CAGAGTA122150.024.76016810-11
Produced by FastQC (version 0.11.5)