FastQCFastQC Report
Fri 10 Feb 2017
ERR1147569.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147569.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852347
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144540.5067405894163648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91010.31907057591520244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69630.24411475882843148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117150.052.3455661
GGTATCA64300.051.4812161
AACGCAG151300.040.299936
TCAACGC158850.038.4219634
ATCAACG161850.037.675013
CAACGCA162300.037.5685775
TATCAAC169050.036.316732
ACGCAGA180300.033.784387
CGCAGAG185300.032.9042978
GTACATG182900.032.1934551
TACATGG189700.030.9835992
GCAGAGT199350.030.704599
ACATGGG189250.030.6171883
CATGGGG106600.028.8504584
ATGGGGG55700.027.7675485
AGAGTAC192400.026.58872610-11
CATGGGT38850.026.1833064
AGTACTT95750.024.22869112-13
CAGAGTA195900.023.8363310-11
ATATGTA19000.023.8146921
Produced by FastQC (version 0.11.5)