FastQCFastQC Report
Fri 10 Feb 2017
ERR1147568.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147568.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863725
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142310.49694017407397706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84890.29643209456215247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63990.22345022654060706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122300.053.6448971
GGTATCA65300.052.3325651
AACGCAG153950.042.2327736
TCAACGC159950.040.648554
ATCAACG160950.040.545953
CAACGCA163200.039.7297175
TATCAAC169500.038.6761972
ACGCAGA180450.035.9958277
CGCAGAG185500.035.208288
GCAGAGT199550.032.759139
GTACATG192450.031.894371
TACATGG195350.031.1221352
ACATGGG196700.030.4246443
CATGGGG103950.028.2690754
TATAACG2154.0472514E-927.6689992
ATGGGGG51850.026.8457935
CATGGGT54850.026.2450774
AGAGTAC192150.026.12693410-11
GAGTACT82900.025.97409412-13
CAGAGTA196700.025.25041210-11
Produced by FastQC (version 0.11.5)