FastQCFastQC Report
Fri 10 Feb 2017
ERR1147567.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147567.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3042167
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175250.5760696240541693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99400.32674077392858447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81810.2689201480392102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131800.057.0938031
GGTATCA67350.052.1520841
AACGCAG172100.043.27586
TCAACGC175000.042.694634
ATCAACG176050.042.4413833
TATCAAC180850.041.676772
CAACGCA179800.041.4224975
ACGCAGA199400.037.260777
CGCAGAG204950.036.221548
GCAGAGT220300.033.8867159
GTACATG195100.031.9482211
ACATGGG200100.030.8295783
TACATGG201850.030.7980652
ATGGGGG53400.030.1890665
CATGGGG107150.029.4242444
AGAGTAC212250.027.67512910-11
TGGGGGG45000.027.363996
CATGGGT48500.027.2290764
TATAACG2853.6379788E-1227.1352882
GTAAGTA24400.026.0873432
Produced by FastQC (version 0.11.5)