FastQCFastQC Report
Fri 10 Feb 2017
ERR1147566.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147566.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3324884
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172070.5175218142948746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104240.31351469705409274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82200.24722667016353053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129650.053.1210981
GGTATCA70900.049.7868461
AACGCAG164150.041.530056
TCAACGC172250.039.7497864
ATCAACG174400.039.2274133
CAACGCA175850.038.766895
TATCAAC187400.036.7918972
ACGCAGA189950.035.927
CGCAGAG197150.034.547848
GCAGAGT209600.032.7227829
GTACATG183900.032.30391
TACATGG189700.031.1401162
ACATGGG190050.030.613243
AGAGTAC199250.028.00378210-11
CATGGGT49900.027.657074
CATGGGG101550.027.6490864
AGTACTT104200.025.23287612-13
CAGAGTA205750.025.16756210-11
GAGTACT100450.024.16141712-13
GTATAGA7900.023.3582761
Produced by FastQC (version 0.11.5)